Table 3.

Signaling pathways associated with differentially expressed proteins as identified by Pathway Studio

Signaling pathways	Overlapping entities	P -value
Metabolic Pathways
Tricarboxylic acid cycle	SUCLA2, PDHB	0.002
Glutathione metabolism	PHGPx, PRDX5	0.011
Cell Signaling Pathways
Adipocytokine signaling	SUCLA2, APOA1, PDHB	0.008
Insulin action	SUCLA2, APOA1, PDHB	0.012

Differentially expressed proteins were entered into Pathway Studio to identify the corresponding signaling pathways that potentially regulate capacitation. Among the differentially expressed proteins (>3-fold) of before- and after-capacitation, at least 5 exhibited regulatory roles in single or more pathways simultaneously (P < 0.05).