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. Author manuscript; available in PMC: 2015 Jan 8.
Published in final edited form as: Endocrinology. 2006 Apr 20;147(9):3995–4006. doi: 10.1210/en.2005-1410

Fig. 1.

Fig. 1

Delayed appearance of IN+ cells in pancreas of Gcgr−/− mice during development. Photomicrographs illustrate confocal microscopic images. A, Pancreas of e10 Gcgr+/+ embryo immunostained for IN shows expression of the hormone. B, Section of pancreas of an e10 Gcgr−/− embryo incubated with IN antibodies demonstrates the absence of immunopositive cells. C, Section of pancreas of an e10 Gcgr−/− embryo incubated with antibodies to PC2 demonstrates the presence of immunopositive cells. Photomicrographs D and E illustrate the presence of PC3/1 in the epithelium (D) and in a cell cluster (E) of e10 pancreatic primordia. Photomicrograph of sections of pancreata of e13.5 (F) and at e15 (G) Gcgr−/− mice and of e15 Gcgr+/+ litter mates (H) illustrates the presence of IN+ cells at these stages of development. Bars, 40 μm.