Fig. 4.

Reappearance of nestin⁺ cells in islets of adult PC2^−/− mice. Photomicrographs of islets from 3-month-old PC2^−/− mice immunostained for visualization of nestin (green) and insulin (red) (A,F), glucagon (red) (C,G), somatostatin (red) (D), or PP (red) (E). A–E: PC2^−/− mice; F–G: PC2^+/+ mice. Coexpressing cells are labeled yellow. Note that nestin is coexpressed by all four endocrine cell types of the islets. B: Pancreas of PC2^−/− mice immunostained for glucagons (red) using a rabbit anti-glucagon sera and a secondary anti-rabbit IgG to visualize islets and a green fluorescent goat anti-mouse IgG (incubation with antibodies to nestin was omitted). Note that nestin cells are not stained. Similar results were obtained with 3-month-old Gcgr^−/− mice. Scale bar = 40 μm.