Fig. 5.

Increased number of nestin⁺ amylase⁺ cells in pancreas of adult Gcgr^−/− mice. A: Low-power photomicrograph of pancreas of 3-month-old Gcgr^−/− mice immunostained for visualization of nestin. Note that nestin is expressed by many exocrine cells and islets (indicated by arrows). B: Pancreas of Gcgr^+/+ mice immunostained for nestin. Note the near absence of nestin-positive cells in exocrine and endocrine tissue. Arrow indicates an unlabeled islet. Scale bar for A and B = 100 μm. C: Photomicrogaph illustrate the presence of scattered nestin⁺ cells in pancreas of Gcgr^+/+. D: In contrast, pancreas of Gcgr^−/− mice contain a large number of nestin⁺ cells. E: Many nestin-positive cells coexpress amylase. Scale bar = 40 μm. Comparable results were obtained with PC2^−/− and PC2^+/+ mice.