Fig. 1.

Fabrication and characterization of PLGA particles. a Modified double emulsion solvent evaporation procedure used for the preparation of Dox/CpG MPs. Dox (red) and CpG (green) solutions were emulsified separately in PLGA dissolved in dichloromethane forming w/o emulsions. The two emulsions were combined and w/o/w emulsion was prepared to obtain PLGA particles co-loaded with Dox and CpG. b SEM microphotographs of Dox, Dox/CpG, and CpG PLGA MPs. The scale bar on the lower right represents 2 μm length. c Percentage Dox and CpG release in PBS (pH 7.4) at 37°C from Dox/CpG MPs (mean ± SEM). Groups were compared using the paired t test (n = 3). d Table representing an average size (diameter) and an average loading (μg/mg particles) of Dox and CpG in different MPs