FIG. 4.

Evolution with time of the proportion of positive PCR for WT and deleted integrase in different virological compartments using specific primers and probes. Positive PCRs were recorded from each experiment using primers or probes specific for WT (W, black bars) and deleted (D, gray bars) integrase. Data are expressed in percent and the number of corresponding positive PCRs over the number of performed PCRs is indicated above or inside each bar. Asterisks correspond to real time PCR with specific probes, while other numbers are from primer-specific PCR. The collection time of samples is indicated in weeks from diagnostic, and samples types are indicated above the bar. Below the A and B graphs are noted, in copies/μl for plasma RNA, and copies/ng of nucleic acid for cellular proviral DNA, the quantitative results obtained by real time PCR for WT and deleted fragments, respectively. PBMC, peripheral blood mononuclear cells.