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Effect of hesperetin on angiogenic signaling. Hesperetin inhibited the phosphorylation of MAPK (A) and PI3K/AKT (B) in VEGF-induced HUVECs. After starvation in EGM without serum overnight, HUVECs were washed twice with PBS and then incubated in the presence of VEGF containing (50 ng/mL) medium with hesperetin for 24 h. β-Actin was used as an internal control.
