Table 3.
Immunohistochemical analysis of in vivo expression of various mycobacterial antigens in the formalin fixed tissues obtained from Mycobacterium tuberculosis -infected lungs and lymph nodes, and non-tuberculous controls
| No. with positive results/total number (%)*** | |||
|---|---|---|---|
| Multibacillary lung tissues** | Tuberculous lymphadenitis | Non-TB controls | |
| ZN staining | 3/3 (100) | 0/17 (0) | 0/12 (0) |
| PCR* | 3/3 (100) | 17/17 (100) | 0/12 (0) |
| Secreted antigens | |||
| MPT32 | 3/3 (100) | 3/17 (18) | 0/12 (0) |
| MPT44 | 1/3 (33) | 4/14 (28) | 6/12 (50) |
| MPT46 | 3/3 (100) | 0/17 (0) | 1/12 (8) |
| MPT51 | 3/3 (100) | 0/17 (0) | 0/12 (0) |
| MPT53 | 3/3 (100) | 3/17 (18) | 1/12 (8) |
| MPT59 | 3/3 (100) | 6/17 (35) | 3/12 (25) |
| MPT63 | 3/3 (100) | 2/17 (12) | 2/12 (17) |
| MPT64 | 3/3 (100) | 17/17 (100) | 2/12 (17) |
| Somatic antigens | |||
| Mce1A | 3/3 (100) | 17/17 (100) | 8/12 (67) |
| Hsp65 | 3/3 (100) | 17/17 (100) | 12/12 (100) |
| MPT57 | 3/3 (100) | 16/16 (100) | 12/12 (100) |
*PCR, N-PCR for amplification of IS6110.
**Vast number of bacilli were detectable by ZN staining.
***A case/control was labelled as positive if any positive signal was recorded in a lesion irrespective of the quality of stain.