Table 4.
Semiquantitative comparative analysis of the various mycobacterial antigens in the individual cases of pulmonary and lymph node tuberculous detected by immunohistochemistry done on formalin fixed paraffin embedded tissues
| Secreted antigens | Somatic antigens | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| MPT32 | MPT44 | MPT46 | MPT51 | MPT53 | MPT59 | MPT63 | MPT64 | Mce1A | Hsp65 | MPT57 | |
| Lung 1 | +++++ | - | +++++ | +++++ | +++++ | ++++ | +++++ | +++++ | +++ | +++++ | ++++ |
| Lung 2 | +++++ | - | +++++ | +++++ | +++++ | ++++ | +++++ | +++++ | +++ | ++ | ++ |
| Lung 3 | +++ | +++ | +++++ | ++++ | ++++ | ++++ | ++++ | ++++ | ++ | ++ | +++ |
| LN 1 | - | - | - | - | - | SNC | - | + | + | +++ | +++ |
| LN 2 | - | SNC | - | - | - | - | - | + | +++ | +++, SNC | +++ |
| LN 3 | - | - | - | - | - | - | - | + | ++ | ++++ | ++++ |
| LN 4 | - | - | - | - | - | - | - | + | + | ++++ | + |
| LN 5 | - | - | - | - | - | - | - | + | +++, SNC | +++++ | +++++ |
| LN 6 | + | - | - | - | - | SNC | - | ++ | ++ | ++++ | ++++ |
| LN 7 | - | - | - | - | - | SNC | - | ++ | ++ | +++ | + |
| LN 8 | - | ND | - | - | SNC | SNC | - | ++ | + | +++ | +++ |
| LN 9 | - | ND | - | - | - | - | - | ++ | ++ | +++ | +++ |
| LN 10 | - | + | - | - | - | - | - | ++ | + | +++ | ++++ |
| LN 11 | - | ND | - | - | - | + | - | ++ | + | +++ | + |
| LN 12 | - | - | - | - | SNC | - | + | ++ | +++ | +++ | +++ |
| LN 13 | + | - | - | - | - | + | - | +++ | + | +++++ | + |
| LN 14 | - | - | - | - | - | - | - | +++ | + | +++ | +++ |
| LN 15 | - | + | - | - | SNC | - | - | +++ | +++ | ++++ | ++++ |
| LN 16 | + | SNC | - | - | - | - | + | +++ | ++ | ++ | ND |
| LN 17 | - | - | - | - | - | - | - | ++ | + | ++ | ++++ |
LN: Lymph node, SNC; staining in the necrotic centres, ND; not determined.
+++++ ~ 80% positive granuloma cells, ++++ ~ 50% positive granuloma cells, +++ 10-25% positive granuloma cells, ++ 2-9% positive granuloma cells, + < 2% positive granuloma cells, - no staining.