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. 2015 Jan 8;11(1):e1004875. doi: 10.1371/journal.pgen.1004875

Figure 6. Developmental regulation of pre-RC components.

Figure 6

Whole cell lysates were prepared from matings between wild type strains, CU427 and CU428, at indicated time points during conjugation. 0–6 h, micronuclear DNA replication; 9–24 h, endoreplication phase I (Endo I). Mating cultures were re-fed at 24 h to complete development (endoreplication phase II; Endo II). Equivalent amounts of total protein (20 µg) were separated by denaturing polyacrylamide gel electrophoresis and subjected to western blot analysis. (B) Flow cytometry analysis samples analyzed in panel A. Nuclei were isolated and stained with propidium iodide. Each histogram represents the number of counted nuclei (x-axis) versus DNA content (y-axis). OM, old parental macronucleus, which is degraded in conjugants. (C) Western blot analysis of wild type cells (CU428) synchronized at the G1/S border by starvation for 18 h or by centrifugal elutriation of a log phase vegetative culture.