Fig. 3.

The involvement of GADD family proteins. The DLD-1 Tet-off POX cells were cultured in medium with 20 ng/ml [(+) DOX], or 100 and 1000 dilution (0.2 ng/ml, 0.02 ng/ml) for 1 or 3 days. The cells were harvested and RNA and cell lysates were prepared and then semi-quantitative RT-PCR (A & B) and western blots (C) for GADD members were performed. Actin was used as control.