Fig. 7.
Effect of Wnt5a on MG63 cell response to surface microstructure and surface energy. MG63 cells plated on TCPS, PT, SLA or modSLA surfaces were cultured with 250 ng ml−1 Wnt5a or 1:200 AbWnt5a until confluence. At confluence, cell number (A), alkaline phosphatase specific activity (B), osteocalcin levels (C) and OPG levels (D) were determined. *p < 0.05, Ti vs. TCPS; #p < 0.05, treatment with either Wnt5a or AbWnt5a vs. untreated MG63 cells on each surface.