Fig. 8.
Effect of Wnt5a on MG63 cell response to surface microstructure and surface energy. MG63 cells plated on TCPS, PT, SLA or modSLA surfaces were cultured with 250 ng ml−1 Wnt5a or 1:200 AbWnt5a until confluence. At confluence, levels of BMP2 (A), BMP4 (B), VEGF (C) and latent TGF-β1 (D) were determined. *p < 0.05, Ti vs. TCPS; #p < 0.05, treatment with either Wnt5a or AbWnt5a vs. untreated MG63 cells on each surface.