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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Jun 16;1843(11):2365–2375. doi: 10.1016/j.bbamcr.2014.06.006

Figure 4.

Figure 4

Effect of 1α,25(OH)2D3 on Wnt5a-dependent PKC activation and effect of Wnt5a in 1α,25(OH)2D3 regulation of PKC activity. (A) MC3T3-E1 cells were treated for 15 minutes with 10−10, 10−9 and 10−8 M 1α,25(OH)2D3. Wnt5a was added to one-half of the cultures at the concentration of 125 ng/ml, at the time of the treatment. At the end of incubation, cell layers were collected for PKC assay. *p<0.05, treatment versus control; #p<0.05, versus 0 M 1α,25(OH)2D3 + vehicle; $p<0.05, versus 0 M 1α,25(OH)2D3 + Wnt5a; @p<0.05 versus 10−10 M 1α,25(OH)2D3 + Wnt5a. (B) MC3T3-E1 cells were treated with 10−8 M 1α,25(OH)2D3 in the presence and absence of 50, 87.5 and 125 ng/ml Wnt5a. At the end of the incubation, the cell layers were collected for PKC assay. *p<0.05, treatment versus control; #p<0.05, versus 0 ng/ml Wnt5a + vehicle; $p<0.05, versus 1α,25(OH)2D3 + 0 ng/ml Wnt5a; @p<0.05 versus 1α,25(OH)2D3 + 5 ng/ml Wnt5a.