Table 3.
Eimeria falcifomis Rhoptry kinases (RopKs)
| gene | Best HMM | Best score | General PK score | E. tenella ortholog | T. gondii ortholog |
|---|---|---|---|---|---|
| EfaB_MINUS_17096.g1521 | ROPK-Eten3 | 252.90 | 95.60 | ETH_00005415, ETH_00028835 | |
| EfaB_MINUS_32658.g2475 | ROPK-Eten5 | 167.00 | 48.30 | ETH_00002510 | |
| EfaB_MINUS_42996.g2710 | ROP35 | 323.90 | 80.10 | ETH_00005905 | TGME49_104740 |
| EfaB_MINUS_720.g57 | ROPK-Unique | 169.60 | 79.70 | ETH_00005190 | ROP18++ |
| EfaB_PLUS_15899.g1411 | ROPK | 146.20 | 86.60 | ||
| EfaB_PLUS_24117.g1969 | ROPK-Unique | 188.30 | 3.00 | ETH_00013325* | |
| EfaB_PLUS_33184.g2393 | ROPK-Unique | 138.40 | 47.10 | ETH_00005170 | |
| EfaB_PLUS_47595.g2679 | ROP21-27 | 367.60 | 90.40 | TGME49_113330 | |
| EfaB_PLUS_7742.g778 | ROP21-27 | 391.80 | 86.80 | ETH_00014495 | TGME49_063220 |
| EfaB_PLUS_8664.g829 | ROP35 | 282.70 | 73.80 | ETH_00026495 |
For putative E. falcifomis RopKs the family categorization obtained searching the HMMs developed by Talevich et al. [49] is given (best HMM). The score against this HMM can be compared with the score against the general protein-kinase-like profile (PKL score) to distinguish RopKs from universal kinases (see also Figure 4). Finally orthologs from E. tenella and T. gondii are given. The E. tenella ortholog marked with * is not included in the RopKs described by Talevich et al. [48], therefore EfaB_PLUS_24117.g1969 is not counted as having a RopK ortholog. Rop18 (marked with ++) is not found in ortholog clusters. The E. falciformis gene, however, shows sequence similarity to Rop18-III from T. gondii (GI: 117574634).