Skip to main content
. 2015 Jan 8;11(1):e1004910. doi: 10.1371/journal.pgen.1004910

Figure 2. Southern blot analysis of meiotic hotspot DSB.

Figure 2

A. Schematic of strains with and without the substrate showing restriction sites and probe locations. B. Meiosis time course of sae2Δ strains without and with the HIS3 CAN1 cassette in the hotspot. Time (hours) after transfer to sporulation media (SPM). C. Schematic of strains with and without the substrate in the coldspot, showing restriction sites and probe locations. D. Meiosis time course of sae2Δ strains without and with the HIS3 CAN1 cassette in the hotspot. Time (hours) after transfer to sporulation media (SPM).