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. 2015 Jan 8;11(1):e1004928. doi: 10.1371/journal.pgen.1004928

Figure 1. Deletion of RAD9 rescues sae2Δ and mre11-D56N cell viability following DSBs through SGS1.

Figure 1

(A–B) Viability of the wild type YMV80 strain and the indicated derivatives plated on YEP+gal. In the presence of galactose, one HO-cut is introduced at leu2 locus (see a scheme in Fig. 2A). For each strain, the number of colonies grown after 3 days at 28°C in YEP+gal was normalized respect YEP+glu. Plotted values are the mean values ± SD from three independent experiments. (C) Exponentially growing cell cultures of the wild type YMV80 strain and the indicated derivatives were serially diluted (1∶10), and each dilution was spotted out into YPD and YPD+camptothecin plates. Plates were incubated 3 days at 28°C.