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. 2015 Jan 8;11(1):e1004923. doi: 10.1371/journal.pgen.1004923

Figure 3. Chromosome rearrangements and delayed replication of a Cre/loxP-mediated deletion (∼5.6 mb) in chromosome 15.

Figure 3

A) Secondary rearrangements of chromosome 15. Mitotic Δ268-5d cells were processed for DNA FISH using a chromosome 15 WCP, and the chromosomal DNA was stained with DAPI. Rearrangements involving chromosome 15 are indicated with arrows. Non-rearranged chromosome 15 s are indicated with asterisks. B and C) BrdU-BAC replication assay on cells containing the ∼5.6 mb distal deletion in chromosome 15. Δ268-5c cells were exposed to BrdU for 4.5 hours, harvested for mitotic cells, stained with an antibody to BrdU (green), and processed for DNA FISH with a chromosome 15 centromeric probe (red) plus a BAC (CTD-2299E17; ΔBAC, red) from the deleted region. The DNA was stained with DAPI (white in panel B or blue in panel C). The red arrows mark the centromeric signals from two chromosomes with DRT/DMC. The white arrows mark the centromeric signals from two non-DRT/DMC chromosome 15 s. The brackets highlight the extreme “pulverization” of two deleted chromosome 15 s.