Figure 1. Effectiveness of intranasal (IN) and intramuscular (IM) L. amazonensis antigens (LaAg) vaccination against subsequent infection with L. braziliensis.

Golden hamsters (6–8 per group) were vaccinated with either 10 µg of LaAg intranasally (•) or 20 µg of LaAg intramuscularly (▴) followed by a booster vaccination 14 days later. Controls received PBS (□). Two weeks after the booster vaccination, animals were infected in the paw with 1×10⁵ L. braziliensis. (A) Lesion thickness was scored on the indicated days. Means ± standard errors of the means (SEM), * p≤0.05, ** p≤0.01. (B) On day 114 post-infection, the lesion thickness of individual feet was shown as individual values, with the horizontal bars representing the median values. Data are from four independent experiments.