Figure 2. Parasite loads from skin lesions of hamsters immunised with crude L. amazonensis antigens (LaAg) and infected with L. braziliensis.

Golden hamsters (4–6 per group) were immunised with IN LaAg (•), IM LaAg (▴), or received PBS (□). Two weeks after the last immunisation, hamsters were challenged with L. braziliensis. A) Paw skin parasite loads were determined 114 days post-infection via a limiting dilution assay. B) Correlation between skin parasite load and lesion thickness (r = 0.70, p<0.0001). Each point represents one animal. The horizontal bars represent the median values, * p≤0.05. Data are from two independent experiments.