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. 2015 Jan 8;11(1):e1004597. doi: 10.1371/journal.ppat.1004597

Figure 1. KapB promotes actin stress fiber formation in human umbilical vein endothelial cells.

Figure 1

Expression of KapB (panel b) or controls (a constitutively active version of MK2 [MK2-EE, panel c], a phosphomimicking version of hsp27 [hsp27-DDD, panel d] or an empty vector control [panel a]) in HUVECs was achieved by retroviral transduction and a 2-day selection with puromycin, after which cells were split onto coverslips and processed for fluorescence microscopy. Cells were fixed in 4% paraformaldehyde (in PBS) permeabilized in 0.1% Triton X-100 (in PBS) and stained with Alexa 555-conjugated to phalloidin to visualize the actin cytoskeleton (panels a-d). MK2-EE and hsp27-DDD have previously been shown to induce actin stress fiber formation in endothelial cells [59]. Scale bar  = 10 µm.