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. 2015 Jan 8;11(1):e1004605. doi: 10.1371/journal.ppat.1004605

Figure 4. The estA2 promoter is repressed by H-NS.

Figure 4

A) The panel shows different PestA2::lacZ fusions. The lacZ gene is shown as a red arrow and the estA2 gene is shown as a blue arrow. PestA2 is illustrated using a bent arrow and the CRP binding site is shown as an orange box. B) H-NS binds to PestA2 only in the presence of flanking DNA. ChIP-PCR was used to measure binding of H-NS to the different PestA2 derivatives cloned in pRW50. PCR products were generated using primers that could detect PestA2 in the context of both the 93 bp fragment and the longer 460 bp fragment. C) The values are β-galactsidase activity values for lysates of M182, or M182Δhns, carrying the different PestA2 derivatives. Assays were done in LB medium.