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. 2015 Jan 8;10(1):e0116566. doi: 10.1371/journal.pone.0116566

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© 2015 Mondal, Pahan

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Spleens of control, EAE (14 dpi) and either cinnamon- or vehicle-treated EAE mice (14 dpi receiving cinnamon/vehicle from 8 dpi) were analyzed for the mRNA expression of Th17-specific factors (RORγt and IL-17) (A). Splenocytes isolated from different groups of mice were analyzed by FACS for RORγt & CD4 (B) and IL-17 & CD4 (C). The MFI of RORγT (D) and IL-17 (E) in CD4+ population were calculated by using the CellQuest software. Data are mean ± SEM of 3 mice per group. ^a p< 0.001 vs normal; ^bp<0.001 vs EAE.