Table 1.

Effect of A2 domain stabilization on specific activities and kinetic parameters

Protein	aPTT, U/µg	Chromogenic assay, U/µg	K1/2 FVIIIa, nM*	K1/2 FIXa, nM*	Km, nM*	Kcat, s-1*
BDD-FVIII	7.00 ± 2.35 (n = 3)	6.87 ± 1.58 (n = 4)	2.08 ± 0.40	1.62 ± 0.62	9.95 ± 0.65	1.48 ± 0.02
D519V/E665V	6.55 ± 1.34 (n = 2)	12.58 ± 2.55 (n = 6)	0.74 ± 0.10	0.43 ± 0.09	11.77 ± 0.82	1.87 ± 0.03

K_cat, constant for catalytic turnover; K_m, Michaelis constant.

^*From kinetic assays. K_1/2, the apparent dissociation constant, is calculated as described in Gale et al.⁵