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. 2014 May 26;33(44):5193–5200. doi: 10.1038/onc.2014.128

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Copyright © 2014 Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/

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Effect of PS2 knockout on the activation of NF-κB, STAT3 and AP-1, and their DNA-binding activities in urethane-induced lung tumors. (a–c) DNA-binding activity of NF-κB (a), STAT3 (b) and AP-1 (c) in the lung tumor tissues was determined by electromobility shift assay in nuclear extracts as described in Materials and methods. (d) Lung tissues were processed and analyzed by immunohistochemistry for detection of positive cells for p50 and p65. (e, f) Tumor extracts were analyzed by western blotting. Samples (20 μg) were resolved on SDS–PAGE and detected with antibodies against p50, p65, IkB and p-IkB (e), and p-STAT3, STAT3, c-Jun and c-Fos (f). β-actin and Histione H1 were used as cytosol marker and nucleus marker, respectively. The experiments shown in Figure 3 were repeated in triplicate with similar results.