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. 2014 May 26;33(44):5193–5200. doi: 10.1038/onc.2014.128

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Copyright © 2014 Macmillan Publishers Limited

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Effect of PS2 knockdown on cell viability, the cleavage of iPLA2, the expression of PRDX6 and activates. (a, b) A549 (a) and NCI-H460 (b) (1 × 106) were transfected with control siRNA and PS2 siRNA for 48 h, and then γ-secretase activity was detected. (c, d) After A549 (c) and NCI-H460 (d) cells were cultured in 96-well plates (1 × 104 cells per well) and transfected with control siRNA and PS2 siRNA for 48 h, cell viability was analyzed by MTT assay. (e, f) The levels of iPLA2 activities were measured in A549 (e) and NCI-H460 (f) transfected with control siRNA and PS2 siRNA for 48 h. (g, h) A549 cell lysates (g) and NCI-H460 cell lysates (h) were analyzed by western blotting. Samples (20 μg) were resolved on SDS–PAGE, and detected with antibodies against iPLA2, PRDX6, PS1, PS2 and β-actin. All values are presented as the mean ±s.e.m. *P<0.05 and **P<0.01, significant difference from the control. The experiments shown in Figure 5 were repeated in triplicate with similar results.