Figure 2.

Enhanced MCT-1 expression induces abscission failure and cell–cell fusion. The PTEN-null MDA-MB-468 cancer cells were analyzed. (a) Time-lapse microscopy was performed and it revealed that the vector control cells entered metaphase (0:40) and completed the mitotic division at 6:00 (h:min). (b) Time-lapse microscopy identified that MCT-1 expression impaired cytokinesis and increased cell–cell fusion, thereby generating giant multinucleated cells. Two typical processes of binucleation were indicated (nos. 1 and 2). (c) The time length in each mitotic stage was analyzed. The MCT-1-overexpressing cells spent longer time in late mitosis from telophase to cytokinesis than the control cells. (d) The cells were activated by the serum for 30 min after starvation for 24 h. Src phosphorylation and p190B expression were enhanced by the ectopic MCT-1 expression (V5-MCT-1). (e) The p190B was IP after the cells treated by nocodazole for 24 h and released for 1 h. The intrinsic/ectopic MCT-1 was co-IP with the active Src. The active phosphorylated p190B and Src were highly induced by MCT-1.