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. 2014 May 26;33(43):5109–5120. doi: 10.1038/onc.2014.125

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Copyright © 2014 Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/3.0/

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MCT-1 promotes multinucleation dependent on the p190B/RhoA function. MDA-MB-468 cells were analyzed. (a) Cells were reactivated for 30 min after starving for 24 h. Immunoprecipitation of p190B was conducted and was it observed that MCT-1 knockdown (shMCT-1) reduced the signal activation and interaction of Src/p190B. (b) RhoA activation and multinucleation were analyzed after depleting p190B by shRNA (nos. 1 and 2). (c) Knockdown of p190B activated RhoA but suppressed the MCT-1-induced multinuclearity. (d) The vectors encoding wtRhoA, caRhoA and dnRhoA were transfected to modify RhoA activity. (e) MCT-1-induced multinucleation was prevented by caRhoA and wtRhoA but promoted by dnRhoA. (f) Overexpression of MCT-1 inhibits PTEN function and works through the Src/p190B/RhoA signaling cascade to induce multinucleation.