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. 2015 Jan 9;5:7697. doi: 10.1038/srep07697

Figure 4. Iso-GNA Caused Cell Death in A549 Cells Was Autophagy-Dependent.

Figure 4

(A) Different concentrations iso-GNA with 0, 2 or 4 mM 3-MA were incubated with A549 cells for 24 h, then cells were stained with trypan blue to test death ratio. (B) Detection of Beclin1 or Atg7 expression by western blot in Beclin1 or Atg7 knock down A549 cells. (C) Different concentrations of iso-GNA were added to normal, Beclin1 knock down or Atg7 knock down A549 cells for 24 h, then trypan blue dye was employed to stain the cells and analyze the death ratio. (D) and (E) Different concentrations of iso-GNA with/without bafilomycin A1 (D) or chloroquine (E) were treated with A549 cells for 24 h, trypan blue dye was employed to stain the cells and analyze the death ratio. Data of three independent tests were shown as means ± s.d. *p < 0.5; **p < 0.01, in comparison with the untreated group. The whole images of western blots are given in Supplementary Figure S8.