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. 2015 Jan 9;5:7697. doi: 10.1038/srep07697

Figure 6. Iso-GNA induced autophagic cell death through the inhibition of Akt/mTOR Pathway.

Figure 6

(A) Western blot was used to detect the p-Akt, Akt, p-mTOR, mTOR, p-p70 S6K, p70 S6K, p-4E-BP1 and 4E-BP1 expression in various concentrations of iso-GNA treated A549 cells. (B) 0, 10 μM of iso-GNA with or without 1 μM rapamycin were added to A549 cells for 24 h, western blot was employed to detect the LC3 expression level. (C) The indicated concentration of iso-GNA with or without 1 μM rapamycin were treated with A549 cells for 24 h, trypan blue dye was employed to stain the cells and analyze the death ratio. Data of three independent tests were shown as means ± s.d. *p < 0.5; **p < 0.01, in comparison with the control group. The whole images of western blots are given in Supplementary Figure S10.