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Tube formation of HRECs after 4 h of incubation in control EBM-2 or EBM-2-based PAM. (A) PAM was diluted at 1/25 (4%, C), 1/10 (10%, D), and 1/4 (25%, E) and compared with control (0%, B). PAM treatment reduced the number of branching points in HREC tube formation dose dependently. (B–E) Representative images of HREC tube formation. Scale bar = 100 μm. **P < 0.01.
