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. 2014 Aug 28;592(Pt 20):4431–4446. doi: 10.1113/jphysiol.2014.276733

Figure 6. Fluorescence recovery after photobleaching (FRAP) quantification of modulation of connexion36 (Cx36) gap junction coupling.

Figure 6

A, percentage recovery in islets treated with 100 μm 18-α-glycyrrhetinic acid (α-GA) or 100 μm mebeverine (Meb) normalized to untreated Cx36+/+ islets. Data represent the mean ± s.e.m. over three mice, with each data point averaged over one to three islets per mouse. B, recovery rate in Cx36+/+ islets either untreated or treated with 50 μm modafinil for 1 h, normalized to untreated islets. Data represent the mean ± s.e.m. over three mice, with each data point averaged over one to three islets per mouse. C, percentage recovery in Cx36+/+ and Cx36−/− islets either untreated or treated with 100 μm 3-isobutyl-1-methylxanthine (IBMX) with 50 μm forskolin (F + IBMX) normalized to untreated Cx36+/+ islets. Data represent the mean ± s.e.m. over three or four mice, with each data point averaged over three or four islets per mouse. For Cx36−/− islets, the mean and s.d. are also presented above the respective data columns. D, recovery rate in C57BL/6 islets cultured in 2 mm, 5 mm or 11 mm glucose for 1 h or 20 mm glucose for 10 min, normalized to recovery rate at 2 mm glucose. Data represent the mean ± s.e.m. over three mice, with each data point averaged over two or three islets per mouse. E, recovery rate in C57BL/6 islets cultured in 2 mm, 5 mm, 11 mm or 20 mm glucose for 24 h, normalized to recovery rate at 2 mm glucose. Data represent the mean ± s.e.m. over three mice, with each data point averaged over two to three islets per mouse. *P < 0.001. NS, no significant difference between conditions.