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. 2014 Aug 28;592(Pt 20):4431–4446. doi: 10.1113/jphysiol.2014.276733

Figure 7. No functional connexion36 (Cx36) gap junction coupling between α cells and β cells in GLU-Venus islets.

Figure 7

A, representative images of unmixed fluorescence for (left to right) Venus, Rhodamine 123 (Rh123), unmixing residuals and the merged final image. Spectra for Rh123 and Venus fluorophores were obtained from Rh123-stained C57BL/6 islets and unstained GLU-Venus islets, respectively. B, representative unmixed images of Rh123 before photobleaching (0 s), Venus expressing α cells before photobleaching, Rh123 immediately after photobleaching (117 s) and Rh123 after fluorescence recovery (322 s) in a GLU-Venus islet. C, percentage fluorescence recovery in α and β cells in a GLU-Venus islet. Data represent the mean ± s.e.m. over three mice, with each data point averaged over four to 24 cells from three to six islets per mouse. D, fraction of total fluorescence intensity recovered in α and β cells of a GLU-Venus islet. Data represent the mean ± s.e.m. fraction over three mice for each time-point, with each data point averaged over four to nine cells from three or four islets per mouse.