Figure 3. p21 overexpression reduces IFN-γ production in B6/lpr-p21tg effector and memory CD4⁺ T cells.

Lymph node CD4⁺ T cells from 4-month-old B6/lpr, B6/lpr-p21tg, B6 and B6-p21tg mice were analyzed by flow cytometry. (a) Intracellular cytokine analysis showed reduced frequency of IFN-γ-producing cells in CD44^hiCD62L^hi CD4⁺ T cell populations from B6/lpr-p21tg compared with B6/lpr mice. Data are from one representative experiment (n = 4 mice; p < 10⁻²) of 2 performed. Percentages of IFN-γ-producing cells were similar for B6-p21tg and B6 CD44^hiCD62L^hi CD4⁺ T cells. (b) The frequency of IFN-γ-producing CD44^hiCD62L^lo CD4⁺ T cells was lower in B6/lpr-p21tg compared with B6/lpr mice. Data are from one representative experiment (n = 4 mice; p < 10⁻²) of 2 performed. Percentages of IFN-γ-producing CD44^hiCD62L^lo CD4⁺ T cells were similar for B6-p21tg and B6 mice. (c) Small CD44^hi cells showed reduced frequency of IFN-γ-producing cells in B6/lpr-p21tg compared with B6/lpr mice. Percentages of IFN-γ-producing small CD44^hi CD4⁺ T cells were similar for B6-p21tg and B6 mice.