Figure 4.
Effect of PS on δ-granule quantal release and release kinetics. (A) PS did not influence the amount of serotonin released from single granules. Similar Trise (B) but higher T1/2, **p = 0.005 (C) values were obtained, indicating slower chemical messenger secretion with enriched PS. (D) The number of granules exocytosed from single platelets did not change. (E) PS increased fusion pore stability, *p = 0.05. (F) Cumulative frequency analysis shows initially fast but later slowed granule trafficking in PS-enriched platelets. (G) Representative amperometric traces from a control platelet (top) and PE-enriched platelet (bottom). Thrombin was applied for 3 s, as indicated by the black bar on traces in part G.