Table 1. Summary of the UPLC–MS/MS Analysis of Each Phospholipid.
phospholipid incubation condition | control | PS | PE |
---|---|---|---|
transition used for relative quantitation | n/a | 812.5 → 208.0 | 718.3 → 577.3 |
injection precision (RSD) | n/a | 5.91% | 15.9% |
RSD of biological replicates | n/a | 23.3 | 13.3 |
total protein in pelleted platelets (μg/mL ± SD) | 117 ± 17 | 113 ± 26 | 118 ± 11 |
percent increase in platelet phospholipid upon incubation (range in 4 replicates) | n/a | 760–874 | 12.9–29.7 |
average percent phospholipid increase (percent ± SD) | n/a | 808 ± 59 | 18.7 ± 7.5 |