Table 2. Determination of Protein Thiols, Nonprotein Thiols, and Total Thiols in Cells Treated with Different Concentrations of NEM.
| NEM concentration (μM) | 0 | 5 | 10 | 20 | 30 | 60 | 200 | 500 |
|---|---|---|---|---|---|---|---|---|
| observed nonprotein thiols (% of control)a | 100% ± 5% | 97% ± 4% | 80% ± 8% | 45% ± 8% | 3% ± 4% | 3% ± 3% | 1% ± 4% | 6% ± 1% |
| observed protein thiols (% of control)a | 100% ± 5% | 103% ± 2% | 88% ± 5% | 72% ± 8% | 43% ± 6% | 29% ± 6% | 12% ± 5% | 7% ± 2% |
| observed total thiols (% of control)a | 100% ± 7% | 95% ± 5% | 81% ± 7% | 54% ± 9% | 28% ± 5% | 21% ± 2% | 9% ± 1% | 6% ± 5% |
| calculated total thiols (% of control)b | 100% ± 5% | 100% ± 3% | 84% ± 7% | 59% ± 8% | 23% ± 5% | 16% ± 5% | 6% ± 5% | 6% ± 2% |
a
n = four repeat wells from the same 96-well plate. Data are presented as percentage of a control in which cells were treated with no NEM. Data are from one representative experiment of a triplicate.
b
Derived from the equation: (A% + B%)/2. A = observed protein thiols; B = observed nonprotein thiols.