Table 3. Total Thiols and Protein Thiols Determined by the High-Throughput Assay vs an HPLC Method.
| NEM concentration (μM) | 0 | 10 | 30 | 200 |
|---|---|---|---|---|
| total thiols | ||||
| high-throughput methoda | 100% ± 5% | 63% ± 3% | 41% ± 2% | 12% ± 6% |
| HPLC | 100% ± 2% | 69% ± 6% | 54% ± 3% | 20% ± 6% |
| protein thiols | ||||
| high-throughput methoda | 100% ± 2% | 89% ± 3% | 68% ± 7% | 20% ± 3% |
| HPLC | 100% | 77% | 60% | 16% |
a
n = three repeat wells from the same plate. Data are presented as percentage of a control in which cells were treated with no NEM. Data are from one representative experiment of a triplicate.