Table 1. Effectiveness of DNA Adduct Enrichment Techniques.
| performance (%)a of extraction with |
||||
|---|---|---|---|---|
| adduct | methanol | 1-butanol | C18 columns | Oasis columns |
| 1,N6-εdA | 8.5 | 24.3 | 15.2 | 90.6 |
| 3,N4-εdCb | ||||
| M1dG | 31.2 | 43.6 | 63.2 | 17.6 |
| N2-FFM-dG | 33.1 | 39.5 | 46.2 | 60.9 |
| N6-FFM-dA | 61.6 | 92.6 | 72.4 | 61.1 |
| N2-MF-dG | 21.9 | 39.3 | 41.5 | 55.2 |
| N6-MF-dA | 15.7 | 19.5 | 22.1 | 23.3 |
| N2-MIE-dG | 51.5 | 63.7 | 56.2 | 75.7 |
| N6-MIE-dA | 43.9 | 48.5 | 55.5 | 39.3 |
| N2-1MIM-dG | 43.8 | 37.4 | 30.2 | 70.3 |
| N6-1MIM-dA | 25.0 | 46.4 | 40.9 | 37.0 |
| C8-PhIP-dG | 88.7 | 38.5 | 2.0 | 14.0 |
| C8-ABP-dG | 32.6 | 31.8 | 29.7 | 46.2 |
| N2-BPDE-dG | 45.6 | 79.0 | 45.6 | 53.2 |
| N2-MP-dG | 51.1 | 54.6 | 34.5 | 54.8 |
| N6-MP-dA | 6.2 | 7.9 | 4.3 | 13.7 |
a
The performance PM,Ad is the residual peak area of the quantifier signal of a particular DNA adduct that remains after processing by one of the workup procedures. The performance PM,Ad (%) was calculated from PM,Ad = RM,Ad × ISM,Ad/100% with the recovery RM,Ad of one of the enrichment methods (Table S-3, Supporting Information) and the remaining signal reduced by ion suppression ISM,Ad (Table S-4, Supporting Information). Details are given in the Experimental Section.
b
The MRM analyses of herring sperm DNA digests showed a substantial background noise in the trace of 255.1 → 139 of the internal standard [15N3]3,N4-εdC that prohibited the evaluation.