Figure 1.

Discovery of the base-susceptible hexasaccharide [1,2,3,1,7] in the MS profiling of the size-fractioned HS oligosaccharide profiles: (A) SEC fractionation of HS oligosaccharides obtained from limited lyase digestion of HSPIM and (B) composition profiles of the hexasaccharide fraction with and without NH₃·H₂O treatment. An unusual HS pentasaccharide with the composition of [1,2,2,1,4] was identified and found to be a base-catalyzed degradation product of a hexasaccharide with the composition of [1,2,3,1,7].