Table 1.
Primers and their melting temperature.
| miR | SNP | Name | Sequence (5′ → 3′) | Tm | Product size (bp) |
|---|---|---|---|---|---|
| 148a | rs6977848 rs199981120 rs185641358 |
miR148a_F miR148a_R |
CTGGCGTCTGGAGCACTG AGGAAGACAGCACGTTTGGT |
58.6 56.9 |
136 |
| 221 | rs113054794 | miR221_F miR221_R |
CCCAGCAGACAATGTAGCTG ACTTGCAAGCTGAACATCCA | 55.9 55 |
93 |
| 186 | rs66461782 | miR186_F miR186_R |
TTGACATTCACATGCTTCAGG CCTTTTGGGCTTTCTGGTTT |
59.7 60.5 |
153 |
| 152 | rs12940701 |
miR152_F miR152_R |
TCTGTCATGCACTGACTGCTC GGGCATGCTTCTGGAGTCTA |
60.2 60.4 |
170 |