Figure 2. Anti-proliferative and pro-apoptotic effects of Ivermectin, Selamectin, and related macrocyclic lactones, and repression of WNT-TCF targets.

1. Chemical structures of Ivermectin and Selamectin. Note that ivermectin is a fermentation mixture with the two major forms denoted by R.
2. Tables of BrdU incorporation IC₅₀s, calculated with PRISM from triplicated data shown in Supplementary Fig S3. Stromectol™ is a commercial name of Ivermectin from the local pharmacy. Results are shown for multiple human cancer types and multiple human colon cancer cells as noted.
3. Table of the percentage of activated Caspase3⁺ cells in different cell types after treatment with different drug concentrations as noted.
4. Heat maps of mRNA expression levels of WNT-TCF targets shown as ratios of housekeeping gene-normalized Ct values from cells under different treatments over normalized Ct values of DMSO-treated control DLD1 or Ls174T cells. No change over control is shown as a value of 1. Dark blue: repression of expression at or below 0.6. Red: enhanced expression above 1.5-fold.
5. Heat map as in (D) showing the changes in the expression for three TCF targets in two colon cancer cell types after treatment with 5 μM Ivermectin, Doramectin, Moxidectin, or Bryostatin for 12 h, compared with DMSO control sibling cells taken at the same time.