Figure 2. APC subtypes in inflamed skin of DKO* mice.

A, B Quantification of flow cytometric analysis of (A) ear epidermal and dermal APCs (n = 7–10), and (B) ear epidermal and dermal APCs after exclusion of LCs and Lan⁺ DDCs. Quantification of MHC-II^lo moDCs, MHC-II⁺ moDCs, CD11b⁺ DCs, and MHC-II⁺ macrophages (n = 3–4).

C Quantification of migratory DCs (CD11c⁺MHC-II^hi cells) in auricular lymph nodes (n = 5–7).

D, E Quantification of (D) ear epidermal and dermal pDCs (CD45⁺CD11c⁺Bst-II⁺B-220⁺CD11b^neg cells) (n = 5–8), and (E) pDCs (CD45⁺CD11c⁺Bst-II⁺B-220⁺CD11b^neg cells) in auricular lymph nodes (n = 6–9) of indicated mice 7–14 days after disease induction measured by flow cytometry.

F Histological ear section stained for Bst-II (green), CD11b (red), and B-220 (blue). Nuclear staining: Hoechst (brown). Arrows indicate double positive (Bst-II/B-220) cells. Scale bar indicates 50 μm.

G Representative images of epidermal ear sheets of indicated mice stained for Langerin. Scale bars indicate 100 μm.

H Epidermal LC numbers counted on ear sheets at the indicated time points. At least three randomly chosen fields were counted for each sample (n = 9–10).

I,J Quantification of (I) ear epidermal (CD45⁺Lan⁺ cells) and dermal (CD45⁺Lan⁺CD103^neg cells) LCs (n = 8–20), and (J) LCs in auricular lymph nodes (Lan⁺CD8^negCD11b⁺CD103^neg) (n = 8–10).

K Quantification of ear dermal Lan⁺ DDCs (CD45⁺Lan⁺CD103⁺ cells) (n = 8–15) of indicated mice 7–14 days after disease induction measured by flow cytometry.

L Representative image of an immunofluorescent staining of an ear section of a day 14 DKO* mouse stained for Langerin (green) and CD103 (red). Arrows indicate double-positive cells, scale bar indicates 100 μm.

M Quantification of Lan⁺ DDCs (Langerin⁺CD8^negCD11b^lo_to_+CD103⁺) in auricular lymph nodes of indicated mice (n = 8–10) by flow cytometry. Data information: Flow cytometric quantifications are depicted as percentage of live cells.

Data represent mean ± SEM. Data were analyzed using unpaired Student's t-test (*P < 0.05, **P < 0.01, ***P < 0.001). P-values for this figure are available in Supplementary Table S3.

Source data are available online for this figure.