Table 3.
Effect of E4 (0.6 or 6 mg/kg/day) treatment on body weight, uterine weight, plasma lipid concentrations, and Oil-red O (ORO) positive area at the aortic sinus in 18-week-old ERα+/+LDLr−/− or ERα−/−LDLr−/− mice
| ERα+/+LDLr−/− |
ERα−/− LDLr−/− |
P, two-factor ANOVA |
|||||||
|---|---|---|---|---|---|---|---|---|---|
| Ctrl (n = 10) | E4 0.6 mg/kg/day (n = 9) | E4 6 mg/kg/day (n = 7) | Ctrl (n = 7) | E4 0.6 mg/kg/day (n = 8) | E4 6 mg/kg/day (n = 4) | Genotype | E4 | Interaction | |
| Body weight (g) | 21.5 ± 0.9 | 18.7 ± 0.6 P = 0.0187 |
16.2 ± 0.3 P < 0.0001 |
20.9 ± 0.8 | 23.2 ± 0.5 | 22.0 ± 1.1 | – | – | P = 0.0004 |
| Uterine weight (mg) | 6 ± 1 | 31 ± 3 P < 0.0001 |
71 ± 7 P < 0.0001 |
3 ± 1 | 4 ± 1 | 6 ± 1 | – | – | P = 0.0001 |
| Total Chol. (mg/dl) | 1152.8 ± 142.2 | 868.4 ± 154.6 | 552.6 ± 44.0 P = 0.0065 |
1102.2 ± 205.3 | 1356.5 ± 124.5 | 1633.3 ± 276.3 | – | – | P = 0.0052 |
| HDL Chol. (mg/dl) | 62.3 ± 9.8 | 77.2 ± 15.1 | 63.7 ± 4.9 | 56.9 ± 14.9 | 61.9 ± 6.2 | 82.6 ± 24.1 | NS | NS | NS |
| ORO area (×103 µm2) | 141 ± 11 | 91 ± 13 P = 0.0369 |
37 ± 5 P < 0.0001 |
133 ± 23 | 140 ± 14 | 151 ± 28 | – | - | P = 0.0028 |
Results were expressed as mean ± SEM. Significance of the observed effects was evaluated using two-way ANOVA. When an interaction was observed between the 2 factors, effect of E4 treatment was studied in each genotype using a Bonferroni's post hoc test (n = 4–8 mice/group)