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. 2014 Sep 19;6(10):1347–1356. doi: 10.15252/emmm.201303604

Figure 4. Therapeutic mimicry of miR-29 attenuates bleomycin-induced fibrosis.

Figure 4

A Hydroxyproline assessment showed a significant increase following bleomycin treatment in both saline- and control-treated groups; however, there was no statistical difference in the miR-29 mimic-treated group between saline- and bleomycin-treated mice. *P < 0.05 (n = 8).

B, C Real-time PCR analysis showed a significant increase of Col1a1 (B) and Col3a1 (C) after bleomycin treatment. miR-29b mimic treatment normalized both Col1a1 and Col3a1 to vehicle-treated expression levels. *P < 0.05 (n = 8).

D Histological examination by trichrome staining showing robust fibrosis in response to bleomycin, which was blunted by miR-29b mimic treatment. Scale bar indicates 50 μm.

E, F Primary pulmonary fibroblasts from patients with IPF were treated with vehicle or TGF-β and transfected with control mimic or miR-29b mimic. Real-time PCR was performed for Col1a1 (E) and Col3a1 (F). miR-29b mimic treatment showed a dose-dependent reduction in both collagens.

G, H A549 cells were treated with vehicle or TGF-β and transfected with control mimic or miR-29b mimic. Real-time PCR was performed for Col1a1 (G) and Col3a1 (H). miR-29b mimic treatment showed a dose-dependent reduction in expression of both Col1a1 and Col3a1.