Table 1.
Primers used for the amplification of the ABCB1 C3435T and CYP2C19*2 polymorphisms.
| Name | Primer sequence (5′ to 3′) | Fragment size (bp) |
|---|---|---|
| ARMS primers | ||
| CYP2C19*2 common | TACGCAAGCAGTCACATAACTAAGC | 205 |
| CYP2C19*2 mutated | TCCCACTATCATTGATTATTTCCCA | |
| CYP2C19*2 normal | TCCCACTATCATTGATTATTTCCCG | |
| ABCB1 C3435T common | CTCACAAGGAGGGTCAGGT | |
| ABCB1 C3435T mutated | GTGGTGTCACAGGAAGAGATT | 278 |
| ABCB1 C3435T normal | GTGGTGTCACAGGAAGAGATC | |
| Sequencing Primers | ||
| CYP2C19*2 | Forward: TCATCTTTGATTCTCTTGTCAGAA | 355 |
| Reverse: | ||
| TAAAGTCCCGAGGGTTGTTG | ||
| ABCB1 C3435T | Forward: | 372 |
| AAGTGTGCTGGTCCTGAAGTT | ||
| Reverse: | ||
| AAGGGTGTGATTTGGTTGC | ||