Figure 3. Overexpression of cardiac MED13 alters liver fatty acid metabolism.
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ALiver from fed MED13cTg mice on normal chow accumulate less lipids than WT, indicated by decreased oil red O staining. Top scale bars, 100 μm; bottom scale bars, 20 μm.
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BMED13cTg liver mitochondria have higher basal oxygen consumption rates, measured using the XF24 Extracellular Flux Analyzer. Data are mean ± SEM, t-test, n = 5.
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C–DMED13cTg liver mitochondria have (C) decreased steady-state levels of short, medium, and long-chain acylcarnitine, and (D) decreased levels of Acyl-CoA species, measured by metabolomics.