Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jan 8;4:185. doi: 10.3389/fcimb.2014.00185

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2015 Vergara, Vidal, Torres and Farfan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Lpf fimbriae involvement in the expression of genes encoding inflammatory molecules. Polarized T84 cells were infected with the EHEC wild-type strain 86-24 and isogenic mutants in lpfA1 (strain CVD468), lpfA2 (strain AGT201), and lpfA1 lpfA2 (strain AGT210). After 3 h of infection, whole cell RNA was extracted to perform real-time RT-PCR analysis for IL8, ICAM1, and CCL20 genes. The fold variation of gene expression was obtained by the comparative cycle threshold (Δ Δ C_T) method, normalized with GAPDH constitutive expression gene.