Table 1.
Oligonucleotide primer pairs used.
| Primer |
||||
|---|---|---|---|---|
| Procedure Gene | Designation | Sequence (5′–3′) | Reference or source | Product size (bp) |
| Resistance genes | ||||
| mef(A) | MEFA1 | AGTATCATTAATCACTAGTGC | Sutcliffe et al. (1996a) | |
| mef(A) | MEFA2 | TTCTTCTGGTACTAAAAGTGG | Sutcliffe et al. (1996a) | 348 |
| tet(O) | TETO1 | AACTTAGGCATTCTGGCTCAC | Olsvik et al. (1995) | |
| tet(O) | TETO2 | TCCCACTGTTCCATATCGTCA | Olsvik et al. (1995) | 519 |
| Φ m46.1 junctions (S. agalactiae chromosome)a | ||||
| SAG0633 | RUMSa-for | GTGTCTGCCTTTCCTTCTGTTGT | This study | |
| mef(A) | MEFA2 | TTCTTCTGGTACTAAAAGTGG | Sutcliffe et al. (1996a) | 3,328 |
| tet(O) | TETO1 | AACTTAGGCATTCTGGCTCAC | Olsvik et al. (1995) | |
| SAG0635 | PHOSa-rev | CTAACAGTAATCGGCTTCTT | This study | 5,994 |
| Φ m46.1 junctions (S. gordonii chromosome)a | ||||
| SGO-1364 | RUMSg-for | GCGAGTTCTCAAAGTCAATAAAA | This study | |
| mef(A) | MEFA2 | TTCTTCTGGTACTAAAAGTGG | Sutcliffe et al. (1996a) | 3,887 |
| tet(O) | TETO1 | AACTTAGGCATTCTGGCTCAC | Olsvik et al. (1995) | |
| SGO-1361 | ADPSg-rev | CTCAGCAACAGCGCAGGTCA | This study | 7,429 |
| Φ m46.1 junctions (S. suis chromosome)a | ||||
| SSUD9_0757 | rum-F | GCATCTCACTTATCCAGCCC | Palmieri et al. (2011a) | |
| mef(A) | MEFA2 | TTCTTCTGGTACTAAAAGTGG | Sutcliffe et al. (1996a) | 3,764 |
| tet(O) | TETO1 | AACTTAGGCATTCTGGCTCAC | Olsvik et al. (1995) | |
| SSUD9_0755 | glf-R | CCTCGTTTCCAGGTCTTCG | Palmieri et al. (2011a) | 7,223 |
| Chromosomal empty targetb | ||||
| SAG0633 | RUMSa-for | GTGTCTGCCTTTCCTTCTGTTGT | This study | |
| SAG0635 | PHOSa-rev | CTAACAGTAATCGGCTTCTT | This study | 1,298 |
| SGO-1364 | RUMSg-for | GCGAGTTCTCAAAGTCAATAAAA | This study | |
| SGO-1361 | ADPSg-R | CTCAGCAACAGCGCAGGTCA | This study | 3,326 |
| SSUD9_0757 | rum-F | GCATCTCACTTATCCAGCCC | Palmieri et al. (2011a) | |
| SSUD9_0755 | glf-R | CCTCGTTTCCAGGTCTTCG | Palmieri et al. (2011a) | 2,597 |
| Φ m46.1 circular form | ||||
| orf1 | ORF1-rev | TAATAAGTGAGAGCAAGTTG | This study | |
| orf63 | ORF63-for | CAGATGGATGGTGTTTCAG | This study | 788 |
| Chromosomal gene used in phage induction experiments (S. pyogenes) | ||||
| speB | SPEB1 | ACCGTGTTATTGTCTATTACC | Banks et al. (2003) | |
| speB | SPEB2 | TGCCTACAACAGCACTTTGG | Banks et al. (2003) | 1,300 |
| Chromosomal gene used in phage induction experiments (S. agalactiae) | ||||
| tkt | tkt-fw | CCAGGCTTTGATTTAGTTGA | Jolley and Maiden (2010) | |
| tkt | tkt-rw | AATAGCTTGTTGGCTTGAAA | Jolley and Maiden (2010) | 859 |
| Chromosomal gene used in phage induction experiments (S. gordonii and S. suis) | ||||
| recA | recA-up | TATGATGAGTCAGGCCATG | King et al. (2002) | |
| recA | recA-dn | CGCTTAGCATTTTCAGAACC | King et al. (2002) | 421 |
| Quantitative real-time reverse transcription-PCR | ||||
| atl | atl-for | TAACAGGTGCGGGTGGAACA | This study | |
| atl | atl-rev | ATCTGACTGACGAGTGGCTT | This study | 206 |
| rDNA16s | P891F | TGGAGCATGTGGTTTAATTCGA | Warwick et al. (2004) | |
| rDNA16s | P1033R | TGCGGGACTTAACCCAACA | Warwick et al. (2004) | 159 |
aFirst primer pair, left junction; second primer pair, right junction.
bFirst primer pair, S. agalactiae; second primer pair, S. gordonii; third primer pair, S. suis.