Table 2.
Lysogenic transfer of Φm46.1: transfer and retransfer assays.
| Transductants |
|||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| MIC (μg/ml)a |
|||||||||||||
| Donor | Recipient | Transfer frequency | Genotype | ERY | TET | ||||||||
| Transfer assaysb | |||||||||||||
| S. pyogenes m46 | S. pyogenes 12RF | 6.0 × 10-4 | mef(A) tet(O) | 16 | 64 | ||||||||
| S. pyogenes m46 | S. agalactiae 1357RF | 6.0 × 10-7 | mef(A) tet(O) | 16 | 64 | ||||||||
| S. pyogenes m46 | S. gordonii 1435RF | 4.3 × 10-6 | mef(A) tet(O) | 16 | 64 | ||||||||
| S. pyogenes m46 | S. suis V36RF | 2.3 × 10-9 | mef(A) tet(O) | 16 | 64 | ||||||||
| S. pyogenes m46 | S. pneumoniae R6 | NDTc | |||||||||||
| S. pyogenes m46 | S. oralis 1235RF | NDT | |||||||||||
| S. pyogenes m46 | S. salivarius 1555RF | NDT | |||||||||||
| Retransfer assays | |||||||||||||
| S. pyogenes 12RF-Td | S. pyogenes 12RF-SN | 1.2 × 10-5 | mef(A) tet(O) | 16 | 64 | ||||||||
| S. agalactiae 1357RF-T | S. pyogenes 12RF-SN | 1.6 × 10-8 | mef(A) tet(O) | 16 | 64 | ||||||||
| S. gordonii 1435RF-T | S. pyogenes 12RF-SN | 2.8 × 10-8 | mef(A) tet(O) | 16 | 64 | ||||||||
| S. suis V36RF-T | S. pyogenes 12RF-SN | 8.5 × 10-4 | mef(A) tet(O) | 16 | 64 | ||||||||
aERY, erythromycin; TET, tetracycline.
bTransfer data from S. pyogenes m46 to S. pyogenes 12RF are from previous experiments (Giovanetti et al., 2003).
cNDT, no detectable transfer.
dThe final T denotes a transductant obtained in the relevant transfer assay.